RT Journal Article
SR Electronic
T1 Detection of rifampin resistant tuberculosis meningitis by polymerase chain reaction linked single strand conformation polymorphism analysis of cerebrospinal fluid
JF Neurosciences Journal
JO Neurosciences (Riyadh)
FD Prince Sultan Military Medical City
SP 105
OP 108
VO 12
IS 2
A1 Ghazvini, Kiarash
A1 Sani, Ashraf T.
A1 Rastegari, Sara
A1 Ghanaat, Javad
A1 Behdani, Mahdi
YR 2007
UL http://nsj.org.sa/content/12/2/105.abstract
AB OBJECTIVE: To rapidly detect rifampin resistance in Mycobacterium tuberculosis isolates causing meningitis in northeast Iran.METHODS: This study presents the results of a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis for the evaluation of rifampin resistance directly from the CSF of 47 patients strongly suspicious to have tuberculosis meningitis in Emam Reza University Hospital, Mashhad, Iran over 3 years (2002 to 2005). Each CSF sample underwent microscopic examination, culture and DNA amplification by 2 PCR protocols and subsequent detection of mutations by SSCP analysis.RESULTS: Among these patients, no mutations were revealed in the rpoB segment by SSCP.CONCLUSION: The SSCP analyses of these samples shows complete susceptibility to rifampin. The use of this method can radically reduce the time needed to provide clinicians with data useful in aiding the selection of appropriate drugs.