RT Journal Article
SR Electronic
T1 Enhanced expression of glial fibrillary acidic protein in the hippocampus one month after whole brain irradiation of adult rats
JF Neurosciences Journal
JO Neurosciences (Riyadh)
FD Prince Sultan Military Medical City
SP 114
OP 119
VO 16
IS 2
A1 Tian, Ye
A1 Yang, Shu
A1 Zhang, Liyuan
A1 Shi, Zhige
A1 Bao, Shiyao
YR 2011
UL http://nsj.org.sa/content/16/2/114.abstract
AB OBJECTIVE: To examine the changes of glial fibrillary acidic protein in the hippocampus after whole brain irradiation of adult rats.METHODS: Forty-one male Sprague-Dawley rats (180-220 g weight and 6-8 weeks) were divided into the 9 irradiation subgroups, sham, and control groups. Their whole brain was irradiated by a single dose of 2, 10, and 30 Gy. At one day, one week, and one month after irradiation, reverse transcriptase-polymerase chain reaction assay was used to semiquantitatively analyze the expression of glial fibrillary acidic protein messenger RNA, and immunohistochemistry was performed to detect glial fibrillary acidic protein-positive cells. This study was carried out at The Second Affiliated Hospital of Soochow University, Jiangsu, China between October 2008 and November 2009.RESULTS: No obvious changes were found between the control and 2 Gy irradiation groups. However, glial fibrillary acidic protein messenger RNA levels were increased after one day and reached a higher level one month after 10 and 30 Gy irradiation. A significant increase in glial fibrillary acidic protein-positive astrocytes was detected one month after 30 Gy irradiation.CONCLUSION: Astrocytes may play an important role in the mechanisms underlying radiation-induced brain injuries in the early stages.